| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Epp, Sandy L (9)
|
MDRAC |
48.60 |
48.02 |
-0.58 |
| 2 |
Banwaree, Saidah N (9)
|
MDPEAK |
51.42 |
50.18 |
-1.24 |
| 3 |
Dumesh, Ariela M (10)
|
MDRAC |
50.33 |
52.55 |
2.22 |
| 4 |
Pruski, Clara C (10)
|
MDRAC |
51.11 |
52.62 |
1.51 |
| 5 |
Farina, Isabella M (10)
|
MDRAC |
54.00 |
54.00 |
0.00 |
| 6 |
Belyansky, Alexandra (10)
|
MDUN* |
1:00.04 |
54.22 |
-5.82 |
| 7 |
Brissett, Gabrielle S (9)
|
MDEST |
53.38 |
54.64 |
1.26 |
| 8 |
Chesnokov, Alexandra (8)
|
MDEST |
55.94 |
54.97 |
-0.97 |
| 9 |
Kelly, Andrea M (10)
|
MDPEAK |
56.10 |
57.75 |
1.65 |
| 10 |
Niles, Riley L (9)
|
MDEST |
56.46 |
1:00.99 |
4.53 |
| 11 |
Din, Nardia K (6)
|
MDEST |
1:23.90 |
1:01.96 |
-21.94 |
| 12 |
Blaha, Sienna K (9)
|
MDEST |
1:01.29 |
1:03.91 |
2.62 |
| 13 |
Rinker, Corinne M (8)
|
MDEST |
1:07.72 |
1:06.48 |
-1.24 |
| 14 |
Wollenweber, Elliana S (10)
|
MDEST |
1:03.14 |
1:07.33 |
4.19 |
| 15 |
Black, Naomi M (9)
|
MDEST |
1:08.49 |
1:15.52 |
7.03 |
| 16 |
Hittle, Ava E (10)
|
MDEST |
1:16.29 |
1:15.85 |
-0.44 |
| 17 |
Hudson, London K (8)
|
MDEST |
|
1:16.07 |
- |
| 18 |
Magden, Macie J (8)
|
MDUN* |
|
1:20.23 |
- |
| 19 |
Duncan, Celeste M (8)
|
MDPEAK |
1:27.22 |
1:27.32 |
0.10 |